immobilized ph gradient (ipg) gel strips (Bio-Rad)
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Immobilized Ph Gradient (Ipg) Gel Strips, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/immobilized+ph+gradient+(ipg)+gel+strips/immobilized+ph+gradient++ipg++strips/pmc10906901-89-2-10
Average 90 stars, based on 1 article reviews
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Incubation:Article Title: A Standard Operating Procedure (SOP) for the preparation of intra- and extracellular proteins of Clostridium acetobutylicum for proteome analysis. Article Snippet: We report on the development of a Standard Operating Procedure (SOP) for extraction and handling of intraand extracellular protein fractions of Clostridium acetobutylicumATCC 824 for reproducible high quality two-dimensional gel electrophoresis (2-DE) analyses.. Standardized cells from a phosphate-limited chemostat were used to evaluate different protein preparation methods.. For the preparation of the secretome, a dialysis/ ultrafiltration procedure resulted in higher protein yields and proved to be more reliable compared to different precipitation methods using TCA, DOC-TCA, acetone, and PEG 6000. |
